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odyssey fc analyzer  (LI-COR)


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    Structured Review

    LI-COR odyssey fc analyzer
    Odyssey Fc Analyzer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 7098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+fc+analyzer/Odyssey+FC+Imaging+System/pmc13109015-696-29-32
    Average 99 stars, based on 7098 article reviews
    odyssey fc analyzer - by Bioz Stars, 2026-10
    99/100 stars

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    Article Title: Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors.
    Article Snippet: After dosing, plates were maintained in tissue culture incubators (37 °C, 5% CO2) for 3 days (WI-38) or 5 days (SCLC cell lines) to allow for at least two cell doublings before processing in an MTT proliferation assay (R&D systems, 4890-050-K) performed according to the manufacturer’s instructions.

    Article Title: Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors.
    Article Snippet: The primary antibodies and dilutions used were as follows: rabbit anti-p-stathmin (Ser38) (Cell Signaling, 3426S, 1:1,000), rabbit anti-total stathmin (Abcam, ab52630), rabbit anti-p-cyclin B (Ser126) (Abcam, ab3488, 1:1,000), rabbit anti-p-nucleolin (Thr84) (Abcam, ab155977, 1:1,000), rabbit anti-p-FOXM1 (Thr600) (Cell Signaling, 14655S, 1:500), rabbit anti-p-RPA2 (Ser33) (Fortis Life Sciences, A300-246A, 1:2,000), rabbit anti-tubulin-HRP (Cell Signaling, 9099S, 1:5,000).

    Article Title: Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity
    Article Snippet: To detect phospho-Stathmin S38, total Stathmin, phospho-Cyclin B1(S126), phospho-nucleolin (T84), phospho-RPA2(S33) and phospho-FOXM1(T600), after incubation with the indicated cyclin RxL inhibitors or DMSO control, NCI-H1048 cells were washed once in ice-cold PBS and lysed in 1% Triton X-100 buffer (40 mM HEPES pH7.5, 1mM EDTA pH 8.0, 10mM sodium pyrophosphate, 10 mM beta-glycerophosphate, 50 mM sodium fluoride, 120 mM sodium chloride and 1% Triton X-100) supplemented with a protease inhibitor cocktail (cOmplete, Sigma #5892970001) and phosphatase inhibitors (PhosSTOP, Sigma #4906837001).

    Incubation:


    Article Title: Vimentin modulates regulatory T cell receptor-ligand interactions at distal pole complex, leading to dysregulated host response to viral pneumonia
    Article Snippet: Membranes were blocked with 5% nonfat milk for 1 h at room temperature, then incubated with primary antibodies against vimentin (ThermoFisher PA1–10003, 1:1000), PKC-θ (clone E1I7Y, Cell Signaling Technology 13643, 1:1000), phospho-PKC-δ/θ (Ser643/676, Cell Signaling Technology 9376, 1:1000), FOXO3a (Invitrogen PA1–805, 1:1000), phospho-FOXO3a (S253, Abcam ab47285, 1:1000), and β-actin (Abcam ab8229, 1:1000) overnight at 4°C. .. After incubation with a goat anti-mouse IRDye 680RD (Li-Cor C50721, 1:5,000) or goat anti-rabbit IRDye 800CW (Li-Cor C60321, 1:10,000) for 1 h at room temperature, the membranes were directly imaged using an Odyssey Fc Analyzer (Li-Cor). .. All figures and statistical analyses were performed using GraphPad Prism 9 software.

    Article Title: Targeting G1–S-checkpoint-compromised cancers with cyclin A/B RxL inhibitors
    Article Snippet: .. Membranes were washed again in TBS-T, incubated with ECL reagent (LI-COR, WesternSure, 92695000) and scanned on the Odyssey Fc Analyzer (LI-COR Biosciences). .. The primary antibodies and dilutions used were as follows: rabbit anti-p-stathmin (Ser38) (Cell Signaling, 3426S, 1:1,000), rabbit anti-total stathmin (Abcam, ab52630), rabbit anti-p-cyclin B (Ser126) (Abcam, ab3488, 1:1,000), rabbit anti-p-nucleolin (Thr84) (Abcam, ab155977, 1:1,000), rabbit anti-p-FOXM1 (Thr600) (Cell Signaling, 14655S, 1:500), rabbit anti-p-RPA2 (Ser33) (Fortis Life Sciences, A300-246A, 1:2,000), rabbit anti-tubulin-HRP (Cell Signaling, 9099S, 1:5,000).

    Article Title: Mitochondrial metabolism and signaling direct dendritic cell function in antitumor immunity
    Article Snippet: .. After washing three times with TBST, the membranes were incubated with 1:5,000-diluted HRP-conjugated anti-mouse IgG (W4021, Promega) for 1 h. Following another three washes, the membranes were imaged by ODYSSEY Fc Analyzer (LI-COR) and AMERSHAM Imager 600 (GE Healthcare). .. The following antibodies were used: anti-ACTB (3700), anti-OPA1 (80471), anti-DRP1 (5391), anti-MFN1 (14739), anti-MFN2 (9482), anti-ULK1 (8054), anti-ULK1-Ser555 (5869), anti-AMPK (2532), anti-AMPK-Thr172 (50081), anti-LC3B (43566) (from Cell Signaling Technology); anti-P62 (18420–1-AP, AB_10694431, Proteintech); anti-NRF1 (GTX103179; GeneTex); total OXPHOS rodent WB antibody cocktail was purchased from Abcam (ab110413).



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    LI-COR odyssey fc analyzer
    Odyssey Fc Analyzer, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+fc+analyzer/Odyssey+FC+Imaging+System/pmc13109015-696-29-32
    Average 99 stars, based on 1 article reviews
    odyssey fc analyzer - by Bioz Stars, 2026-10
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